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Covalent Self-labeling of Tagged Proteins with Chemical Fluorescent Dyes in BY-2 Cells and Arabidopsis Seedlings
BY-2 cells Arabidopsis SNAP-tag live cell imaging microtubules endocytosis PIN2 auxin transporter
2023/12/21
Synthetic chemical fluorescent dyes promise to be useful for many applications in biology. Covalent, targeted labeling, such as with a SNAP-tag, uses synthetic dyes to label specific proteins in vivo ...
p24 family proteins are involved in transport to the plasma membrane of GPI-anchored proteins in plants
Arabidopsis p24 family endoplasmic reticulum glycosylphosphatidylinositol (GPI)
2023/12/19
p24 proteins are a family of type-I membrane proteins which cycle between the endoplasmic reticulum (ER) and the Golgi apparatus via Coat Protein I (COPI)- and COPII-coated vesicles. They have been pr...
Lipid Signaling through G Proteins
N-acylethanolamines G proteins lipid signaling endocannabinoids
2023/12/11
N-Acylethanolamine (NAE) signaling has received considerable attention in vertebrates as part of the endocannabinoid signaling system, where anandamide acts as a ligand for G protein–coupled cannabino...
Mapping and engineering of auxin-induced plasma membrane dissociation in BRX family proteins
BRX BREVIS RADIX (BRX) phloem development auxin-responsive biological activity
2023/12/4
Angiosperms have evolved the phloem for the long-distance transport of metabolites. The complex process of phloem development involves genes that only occur in vascular plant lineages. For example, in...
The Ca2+ sensor proteins CML37 and CML42 antagonistically regulate plant stress responses by altering phytohormone signals
Jasmonates Abscisic acid Drought Herbivory Necrotrophic pathogenes Defense Calcium Glucosinolates Spodoptera littoralis Alternaria brassicicola
2023/12/1
A transient increase in the cytosolic calcium concentration is one of the first reactions that can be measured in plant cells upon abiotic as well as biotic stress treatments. These calcium signals ar...
Arabidopsis HIPP proteins regulate endoplasmic reticulum-associated degradation of CKX proteins and cytokinin responses
proteins regulate Endoplasmic reticulum (ER) CKX proteins plant development
2023/12/1
Eukaryotic organisms are equipped with quality-control mechanisms that survey protein folding in the endoplasmic reticulum (ER) and remove non-native proteins by ER-associated degradation (ERAD). Rece...
BBX19 fine-tunes the circadian rhythm by interacting with PSEUDO-RESPONSE REGULATOR proteins to facilitate their repressive effect on morning-phased clock genes
BBX19 circadian clock transcription factors zinc Arabidopsis thaliana
2023/12/1
The core plant circadian oscillator is composed of multiple interlocked transcriptional–translational feedback loops, which synchronize endogenous diel physiological rhythms to the cyclic changes of e...
Quantitative proteome analysis reveals changes of membrane transport proteins in Sedum plumbizincicola under cadmium stress
proteome Sedum Quantitative proteome analysis
2023/6/2
plumbizincicola is an herbaceous species tolerant of excessive cadmium accumulation in above-ground tissues. The implications of membrane proteins, especially integrative membrane proteins, in Cd deto...
SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression
SaHsfA4c Sedum alfredii ROS-Scavenger Proteins Expression
2023/6/5
The heat shock transcription factor (Hsf) family, an important member in plant stress response, affects cadmium (Cd) tolerance in plants. In this study, we identified and functionally characterized a ...
Two Chloroplast Proteins Negatively Regulate Plant Drought Resistance Through Separate Pathways
Chloroplast Proteins Negatively Plant Drought Resistance OST1-dependent OST1-independent
2024/2/4
Drought is one of the most deleterious environmental conditions affecting crop growth and productivity. Here we report the important roles of a nuclear-encoded chloroplast protein, PsbP Domain Protein...
A role for plant KASH proteins in regulating stomatal dynamics
plant KASH proteins stomatal dynamics Protein complexes nuclear envelope cytoskeleton
2024/2/5
Stomatal movement, which regulates gas exchange in plants, is controlled by a variety of environmental factors, including biotic and abiotic stresses. The stress hormone abscisic acid (ABA) initiates ...
The use of cotyledon proteins to assess the genetic diversity in sweet holm oak
seed storage proteins genetic resources sweet holm oak
2015/3/16
Sweet holm oak (Quercus ilex ssp. ballota Desf. Samp.) is an important broad-leaved tree spread in the Mediterranean basin. In Spain, few studies on the genetic variability of this species have been d...