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Change in the Amount of Amino Nitrogen in Crown Root of Sugar Beet (Beta vulgaris L.) Infected with Rhizoctonia solani
Amino nitrogen Rhizoctonia root rot resistance Sucrose Sugar beet Sugar quality
2009/7/8
The α-amino nitrogen (A-N) contained in the roots and crowns of sugar beet is one of the harmful non-sugars that deteriorate the quality of sugar beet roots. This research was conducted to investigate...
pCAMBIA2300-betA-BADH双价基因植物表达载体的构建
betA BADH 酶切 PCR
2009/5/11
本实验以载体pCAMBIA2300-35s-OCS为基础通过分子克隆手段,将甜菜碱合成途径的两个关键酶基因,即编码胆碱脱氢酶(CDH)的betA基因和编码甜菜碱醛脱氧酶(BADH)的BADH基因及启动子和终止序列构建在同一植物表达载体上,得到pCAMBIA2300-betA-BADH双价植物表达载体。并将其导入根癌农杆菌GV3101和LBA4404,进一步用于双子叶植物的遗传转化。
Transfer of a b-Glucuronidase Reporter Gene to Sugarbeet(Beta vulgaris L.)Via Microprojectile Bombardment
b-Glucuronidase Reporter Gene Sugarbeet(Beta vulgaris L.) Microprojectile Bombardment
2009/1/14
For the transfer of a b -Glucuronidase (GUS) reporter gene to sugarbeet (Beta vulgaris L.) callus and leaf explants by microprojectile bombardment, various rupture disk pressures and sample plate dis...