搜索结果: 1-4 共查到“生物物理学 FRET”相关记录4条 . 查询时间(0.078 秒)
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2017年6月19日,英国皇家化学会主办刊物Chemical Communications(ChemComm)以封面论文(Inside Front Cover)的形式在线发表了中国科学院生物物理研究所柯莎(Sarah Perrett)研究组题为A Co-expression Strategy to Achieve Labeling of Individual Subunits within a D...
cAMP依赖的蛋白激酶( protein kinase A, PKA)在细胞生长与分化过程中扮演重要角色,特别是在调节Ras信号通路引起的细胞增殖效应中起着重要作用。为了在活细胞内动态观察表皮生长因子(epidermal growth factor, EGF)对PKA的作用,采用一种可以检测PKA酶活性的报告蛋白(A-kinase activity reporter, AKAR)———这种报告蛋白...
利用FRET技术在活细胞内研究红景天甙对Aß25-35诱导PC12细胞凋亡的抑制作用
红景天甙(Salidroside) PC12细胞(PC12 cells) 细胞凋亡(apoptosis) Aß 25-35 荧光共振能量转移(FRET)(Fluorescence Resonance Energy Transfer (FRET))
2008/5/19
为研究红景天甙(Salidroside)对ß淀粉样肽25-35(ß amyloid peptide25-35, Aß25-35)诱导PC12细胞凋亡的抑制作用,本文采用Cell Counting Kit-8 (CCK-8)分析细胞的存活率,通过光镜检测细胞形态以及Hoechst染色检测细胞核固缩,利用荧光共振能量转移(Fluorescence Resonance...
Rho small GTPase regulates the stability of individual focal adhesions: a FRET-based visualization of GDP/GTP exchange on small GTPases
cell motility cell-substratum adhesion Rac
2010/12/2
RhoA and Rac1 are small GTPases primarily involved in cytoskeletal remodeling. Many biochemical studies have suggested that they are also key organizers of cell-substrate adhesion. Recently, fluoresce...