搜索结果: 1-15 共查到“NADPH”相关记录43条 . 查询时间(0.062 秒)
Persulfidation-based Modification of Cysteine Desulfhydrase and the NADPH Oxidase RBOHD Controls Guard Cell Abscisic Acid Signaling
Abscisic Acid Signaling NADPH oxidase Persulfidation Guard cells
2024/1/22
Accumulating evidence suggests that hydrogen sulfide (H2S) is a gaseous signaling molecule that regulates diverse cellular signaling pathways through persulfidation, which involves the post translatio...
NADPH（还原型辅酶Ⅱ）是细胞内关键的抗氧化分子，它参与生物体内还原性生物合成以及氧化还原反应，在代谢通路中发挥重要作用。但细胞如何感知NADPH水平的变化以及NADPH是否具有其他生理功能等仍有待阐明，需要进一步探索。
通常情况下，细胞内有三个主要的NADPH生成途径：磷酸戊糖途径、叶酸代谢途径和苹果酸酶代谢途径。长期以来的研究表明，NADPH作为细胞内关键的抗氧化分子，参与生物体内还原性生物合成以及氧化还原反应。但是细胞如何感知NADPH水平的变化以及NADPH本身的生理功能一直没有得到关注。2021年1月18日，中国医学科学院基础医学研究所杜文静教授课题组联合清华大学生命学院薛毅研究员课题组在Nature M...
NADPH氧化酶家族在心血管疾病中作用的研究进展
活性氧 烟酰胺腺嘌呤二核苷酸磷酸氧化酶 结构 心血管疾病
2020/2/12
烟酰胺腺嘌呤二核苷酸磷酸氧化酶是心血管系统中活性氧(reactive oxygen species，ROS)的主要来源，它包括7种亚型并分别表达在不同的心血管细胞及其细胞器中，参与调节细胞增殖、迁移、分化、凋亡、衰老和炎症反应等多种活动，其衍生的ROS参与高血压、动脉粥样硬化、糖尿病血管病变、心肌梗死后心室重构等多种心血管疾病的病理过程。
目的 从甘草Glycyrrhiza uralensis中克隆1个新的NADPH细胞色素P450还原酶基因（GuCPR，登录号：MH401048）并进行生物信息学分析。方法 提取甘草根部总RNA后逆转录为cDNA，通过转录组数据库筛选得到GuCPR基因全长，利用NCBI ORF finder得到其开放阅读框并翻译得氨基酸序列，设计引物进行PCR扩增，构建克隆重组质粒。利用生物信息分析预测蛋白性质、结...
NADPH氧化酶在1-戊烯-3-酮诱导的拟南芥早期信号事件中的作用
拟南芥 1-戊烯-3-酮 NADPH氧化酶 非损伤微测技术
2018/11/27
1-戊烯-3-酮(1-penten-3-one)作为重要的植物挥发性信号物质可诱导拟南芥防御反应早期信号的产生。但是拟南芥叶肉细胞响应1-戊烯-3-酮早期信号转导途径尚未见到报道。本研究以拟南芥为材料, 研究了NADPH氧化酶对1-戊烯-3-酮处理后拟南芥早期信号的作用。实验采用非损伤微测技术结合激光共聚焦技术探究了1-戊烯-3-酮诱导的活性氧(ROS)、跨膜离子流信号与NADPH氧化酶之间的关系...
DISTINCT LOCALIZATION OF NADPH OXIDASE FLAVOCYTOCHROME B IN RESTING AND INTERFERON GAMMA ACTIVATED MACROPHAGES
Host defense superoxide
2015/5/21
Flavocytochrome b558, the catalytic core of the phagocytic NADPH oxidase, mediates the transfer of electrons from NADPH to molecular oxygen to generate superoxide for host defense. Flavocytochrome b i...
A Fluorescently Tagged C-Terminal Fragment of p47phox Detects NADPH Oxidase Dynamics during Phagocytosis
NADPH Oxidase Dynamics phagocytosis
2014/12/12
The assembly of cytosolic p47phox and p67phox with flavocytochrome b558 at the membrane is crucial for activating the leukocyte NADPH oxidase that generates superoxide for microbial killing. p47phox a...
FcγR-stimulated activation of the NADPH oxidase: phosphoinositide-binding protein p40phox regulates NADPH oxidase activity after enzyme assembly on the phagosome
NADPH oxidase p40phox
2014/12/12
The phagocyte NADPH oxidase generates superoxide for microbial killing, and includes a membrane-bound flavocytochrome b558 and cytosolic p67phox, p47phox, and p40phox subunits that undergo membrane tr...
Rac Activation Induces NADPH Oxidase Activity in Transgenic COSphox Cells and Level of Superoxide Production is Exchange Factor-Dependent
NADPH oxidase Rac
2014/12/12
Transient expression of constitutively active Rac1 derivatives, (G12V) or (Q61L), was sufficient to induce phagocyte NADPH oxidase activity in a COS-7 cell model in which human cDNAs for essential oxi...
Rac GTPase isoform-specific regulation of NADPH oxidase and chemotaxis in murine neutrophils in vivo: Role of the C-terminal polybasic domain
Rac GTPase NADPH oxidase
2014/12/12
The Rho family GTPase Rac acts as a molecular switch for signal transduction to regulate various cellular functions. Mice deficient in the hematopoietic-specific Rac2 isoform exhibit agonist-specific ...
The phosphoinositide-binding protein p40phox activates the NADPH oxidase during FcγIIA receptor–induced phagocytosis
NADPH oxidase phagocytosis
2014/12/12
Superoxide produced by the phagocyte reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is essential for host defense. Enzyme activation requires translocation of p67phox, p47phox, an...
NADPH氧化酶是一种与哺乳动物嗜中性粒细胞gp91phox同源的氧化还原酶，主要参与植物的防御反应，并调节植物的生长发育。当植物受到生物或非生物胁迫时，该酶会大量产生活性氧，使植物及时对逆境胁迫做出反应，以适应外界环境的变化。尽管对该蛋白的功能已有不少研究报道，但其在活细胞中参与逆境胁迫的机制尚不清楚。
探讨熊果酸（ursolic acid，UA）对大鼠肝星状细胞（HSC-T6）的NADPH氧化酶（NOX）-Hedgehog（Hh）信号通路的影响。 方法 将处于对数生长期的HSC-T6细胞分为6组：正常对照组、瘦素组（100 ng/mL）、熊果酸自身对照组（50 μmol/L）、DPI自身对照组（20 μmol/L）、熊果酸干预组（瘦素+熊果酸）、DPI干预组（瘦素+DPI...