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Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants
Agrobacterium tumefaciens genetic engineering GUS activity Picea abies Karst
2015/3/27
In conifers and other plants with long reproductive cycles, transformed embryogenic tissues can serve as a convenient source of plant material for the testing of insecticidal or fungicidal transgene e...
Effects of different sources of iron, hormones and Agrobacterium tumefaciens on the chlorophyll and iron concentration in the leaves of peach trees
acidic compounds hormones iron chlorosis peach cultivars rootstocks
2015/1/22
The aim of this study was to investigate the effect of different sources of iron, hormones and Agrobacterium tumefaciens in the chlorophyll and iron concentration of the peach cultivars Katerina and F...
An improved Agrobacterium tumefaciens mediated transformation of Artemisia annua L. by using stem internodes as explants
anti-malaria artemisinin stem internode traditional Chinese medicine transgene
2014/2/24
Transformation of Artemisia annua, which produces the sesquiterpenoid endoperoxide artemisinin widely used for the treatment of malaria, has been hampered by the low efficiency of adventitious shoot a...
Transformation of HBsAg (Hepatitis B Surface Antigen) Gene into Tomato Mediated by Agrobacterium tumefaciens
Agrobacterium HBsAg gene tomato transformation
2014/2/25
The plant expression vector pBRSAg was constructed as suitable for transformation via Agrobacteriummediated approach. It contains all elements for plantexpression, such as CaMV 35S promoter, both left...
The effect of different antibiotics on the elimination of Agrobacterium and high frequency Agrobacterium-mediated transformation of indica rice (Oryza sativa L.)
Agrobacterium-mediated transformation Agrobacterium elimination gus reporter gene hygromycin phosphotransferase selectable marker gene (hpt) rice
2014/2/25
We report here the suitable explant with high efficiency of transformation and the positive effects of timentin over other antibiotics like carbenicillin and cefotaxime on the elimination of Agrobacte...
Agrobacterium Tumefaciens Nitrogen Fixation System Expresses in Sinorhizobium meliloti (=Rhizobium meliloti)
Acetylene reduction dry weight root nodules Alfalfa Sinorhizobium meliloti Rhizobium meliloti Agrobacterium tumefaciens
2010/11/16
Kanvinde and Sastry (Appl. and Environ. Microbiol. 56:2087-2092, 1990) had previously
demonstrated that A. tumefaciens has all the physiological characters of a diazotroph. Genetic evidence indicates...
Rooting Induction of Soft Wood and Almond Micro-cutting Using Agrobacterium Rhizogenes
rooting induction almond micro cutting Agrobacterium rhizogenes
2010/11/15
The present work was carried out during the period between 1999 till 2003 in the laboratories
of Agriculture Development System (ADS). Cairo, Egypt. This study aimed to investigate rooting induction ...
Agrobacterium-Mediated Transformation in Liliaceous Ornamental Plants
Faculty of Agriculture,Niigata University,Japan
2009/9/10
Studies on Agrobacterium-mediated transformation in 3 Liliaceous ornamental plants, Lilium formosanum, Agapanthus praecox ssp. orientalis and Muscari armeniacum, were described. Three different strain...
Antibiotics for Agrobacterium Elimination in Apple Transformation
cefotaxime Malus × domestica Borkh. meropenem shoot regeneration
2010/11/23
We investigated several antibiotics including carbenicillin (CBPC), clavulanic acid/amoxicillin (CVA/AMPC), cefotaxime (CTX), meropenem (MEPM), vancomycin (VCM) and doxycycline (DOXY) for Agrobacteriu...
Tissue Culture-independent In Planta Transformation Strategy：an Agrobacterium tumefaciens-Mediated Gene Transfer Method to Overcome Recalcitrance in Cotton (Gossypium hirsutum L.)
Planta Transformation Strategy Agrobacterium tumefaciens-Mediated Gene Transfer Method Cotton
2009/4/30
The major constraint in cotton improvement has been the recalcitrance of cotton varieties to tissue culture. Alternate methods that avoid/minimize tissue culture would be beneficial for the improvemen...
Agrobacterium-mediated Transformation of Broad Bean Vicia faba L.
Vicia faba L., Agrobacterium tumefaciens Agrobacterium rhizogenes peroxidase
2009/3/23
Three broad bean cultivars, which varied in morphology and geographical origin, were inoculated with nine Agrobacterium strains to determine the best combination for potential use in transformation an...
Genetic Transformation of Coleus blumei Benth.Using Agrobacterium
callus culture Coleus blumei transformation Agrobacterium
2009/3/20
Efficiency in transformation of three hybrids of Coleus blumei using wild and mutant strains of Agrobacterium sp. was evaluated. Successful transformation depended on specific bacterial strain-plant g...
A new hemA gene encoding 5-aminolevulinate （ALA） synthase was cloned from Agrobacterium radiobacter zju-0121. The ALA synthase catalyzes the pyridoxal phosphate-dependent condensation of succinyl coen...
Agrobacterium tumefaciens mediated transformation and regeneration of ginger（Zingiber officinale Rosc）
Genetic transformation b-glucuronidase GUS expression
2009/2/19
The absence of seed set in ginger makes conventional breeding methods inapplicable warranting genetic modification through biotechnological means. Agrobacterium tumefaciens strain EHA105/p35SGUSIn...
Detection of Crown Gal Agent（Agrobacterium tumefaciens）in Grapevine Propagating Material
Crown Gal Agent Grapevine Propagating Material
2009/1/23
Polyclonal antiserum produced against Agrobacterium tumefaciens (biovar 3) reacted in immunofluorescent test with biovar 3 strains of A.tumefaciens,causal agent of crown gal, from Central Anatolia. T...