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Microsurgical nuclear transfer by intraooplasmic karyoplast injection as an alternative embryo reconstruction method in somatic cloning of pigs and other mammal species; application value of the method and its technical advantages: a review
somatic cloning microsurgical nuclear transfer karyoplast intraooplasmic injection chromatin remodelling epigenetic reprogramming
2015/6/1
The introduction of cell nuclei into enucleated recipient cells, beyond enucleation, is the most significant stage of somatic cloning procedure. Microsurgical transfer of somatic nuclei can be an alte...